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一种用于微蛋白鉴定和序列分析的综合方法
JoVE 신문
생물학
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JoVE 신문 생물학
An Integrated Approach for Microprotein Identification and Sequence Analysis
DOI:

09:37 min

July 12, 2022

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Chapters

  • 00:04Introduction
  • 00:40Loading the Phylogenetic Codon Substitution Frequencies (PhyloCSF) Track Hub to the University of California Santa Cruz (UCSC) Genome Browser
  • 01:25Navigating to Genes of Interest Using Gene Identifiers
  • 02:20Navigating to Genomic Regions of Interest Using Sequence Information
  • 03:10Identifying Conserved Short Open Reading Frames (sORFs) Using PhyloCSF Track Data
  • 04:06Viewing Homologous Regions in Other Genomes
  • 04:51Generating Multi-Species Sequence Alignments for Microproteins of Interest
  • 06:47Results: Identification of Genomic Regions of Interest for Microprotein-Coding Potential
  • 08:47Conclusion

Summary

자동 번역

这里描述的协议提供了有关如何在用户友好的UCSC基因组浏览器上使用PhyloCSF分析微蛋白编码潜力的感兴趣基因组区域的详细说明。此外,建议使用几种工具和资源来进一步研究已鉴定的微蛋白的序列特征,以深入了解其假定的功能。

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