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Geavanceerde beeldvorming met hoge resolutie van virusassemblages in vloeistof en ijs
JoVE 신문
생화학
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JoVE 신문 생화학
Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
DOI:

08:31 min

July 20, 2022

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Chapters

  • 00:05Introduction
  • 01:43Loading the Specimen Holder for Liquid-EM
  • 02:47Production of Microchip Sandwich Assemblies
  • 03:51Imaging Specimens Using a Transmission Electron Microscope
  • 05:15Analysis of SARS-CoV-2 Sub-Viral Assemblies in Liquid
  • 06:09Results: Representative structures of AAV, SARS-CoV-2 Sub-Viral Assemblies and Rotavirus DLPs Prepared in Liquid and Vitreous Ice Using the Microchip Sandwich Technique
  • 07:24Conclusion

Summary

자동 번역

Hier worden protocollen beschreven om virusassemblages voor te bereiden die geschikt zijn voor vloeistof-EM- en cryo-EM-analyse op nanoschaal met behulp van transmissie-elektronenmicroscopie.

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