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Chapters
- 00:05Introduction
- 01:03C. elegans Culturing on OP50
- 04:05Preparing Gut Microbiota Isolate Collections for Re-Feeding Worms
- 05:14LFASS Heat Shock and Oxidative Assay Setup
- 07:49Data Inspection
- 08:26LFASS Data Processing
- 10:02Results: High-Throughput Screening of Bacterial Isolates for Impact on Nematode Stress Resistance
- 10:47Conclusion
Gut microbes may positively or negatively impact the health of their host via specific or conserved mechanisms. Caenorhabditis elegans is a convenient platform to screen for such microbes. The present protocol describes high-throughput screening of 48 bacterial isolates for impact on nematode stress resistance, used as a proxy for worm health.
