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1Putuo People’s Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology,Tongji University, 2National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics,Chinese Academy of Sciences, 3Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital,Fudan University
Chapters
- 00:04Introduction
- 00:44Preparation of Myelin Oligodendrocyte Glycoprotein Peptide (MOG35-55) Emulsion and Pertussis Toxin
- 01:55Establishment of Experimental Autoimmune Encephalomyelitis (EAE) Animal Model
- 02:55Open Field Test
- 03:42Analysis of Bone Phenotype
- 04:16Results: Induction of EAE by MOG35-55 Treatment
- 05:49Conclusion
El presente protocolo describe la inducción de encefalomielitis autoinmune experimental en un modelo de ratón utilizando glicoproteína oligodendrocitos de mielina y monitoreando el proceso de la enfermedad utilizando un sistema de puntuación clínica. Los síntomas experimentales relacionados con la encefalomielitis autoinmune se analizan mediante análisis de tomografía microcomputarizada de fémur de ratón y prueba de campo abierto para evaluar el proceso de la enfermedad de manera integral.
