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自下而上的 体外 方法测定隔膜的超微结构组织、膜重塑和曲率敏感性行为
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JoVE 신문 생물학
Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
DOI:

09:09 min

August 17, 2022

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Chapters

  • 00:05Introduction
  • 00:49Designing Wavy NOA (Norland Optical Adhesive) Replica from Wavy PDMS (Polydimethylsiloxane) Patterns
  • 01:49Generation of a Supported Lipid Bilayer and Protein Incubation
  • 03:22Sample Fixation, Sample Dehydration, Critical Point Drying
  • 04:43Sample Mounting and Coating
  • 05:54Acquire the Images Using a Field-Emission SEM (FE-SEM) Microscope
  • 06:56Results: Analysis of the Self-Assembly of Septins at the Lipid Membrane
  • 08:27Conclusion

Summary

자동 번역

败类是细胞骨架蛋白。它们与脂质膜相互作用,可以感测但也会产生微米级的膜曲率。我们在该协议中描述了用于分析膜变形,曲率敏感的隔膜结合和隔膜长丝超微结构的自下而上的 体外 方法。

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