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水性抗生素耐药细菌的分离和鉴定及其抗生素耐药基因的分子表征
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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
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水性抗生素耐药细菌的分离和鉴定及其抗生素耐药基因的分子表征

DOI:
10.3791/63934-v

•

08:58 min

•

March 03, 2023

•

,
1Antimicrobial Research (AMR) Lab, Department of Biotechnology,University of Mumbai

Chapters

  • 00:04Introduction
  • 01:09Estimation of Total Bacterial Load and Antibiotic-Resistant Bacteria Count
  • 02:33DNA Template Preparation and PCR Amplification for 16S rRNA Gene Sequencing
  • 04:05Detection of Antibiotic Resistance in the Isolates by Antibiotic Susceptibility Testing
  • 06:08Results: Identification of Antibiotic-Resistant Bacteria and Their Resistance Genes
  • 08:04Conclusion

Summary

자동 번역

자동 번역

在这里，我们提出了一个详细的方案，用于从水中分离和鉴定抗生素耐药细菌及其抗生素抗性基因（ARG）的分子特征。使用基于培养物和非基于培养物（宏基因组分析）技术提供了有关印度孟买淡水中存在的细菌多样性和不同ARG的总库的完整信息。

Tags

Antibiotic-resistant BacteriaWater SampleCulture-based TechniquesCulture-independent TechniquesTotal Bacterial LoadColony Forming UnitsAntibiotic Resistance GenesPCRMolecular Characterization

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