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1Department of Neuroanatomy,Juntendo University Graduate School of Medicine, 2Department of Cell Biology and Neuroscience,Juntendo University Graduate School of Medicine, 3Advanced Research Institute for Health Sciences,Juntendo University, 4Department of Neuroscience, Graduate School of Medicine,Kyoto University, 5Japan Society for the Promotion of Science, 6Department of Oral Anatomy and Neurobiology, Graduate School of Dentistry,Osaka University, 7Department of Multi-Scale Brain Structure Imaging,Juntendo University Graduate School of Medicine
Chapters
- 00:04Introduction
- 00:57Tissue Clarification
- 02:06Brain Slice Mounting
- 03:52Confocal Laser Scanning Microscopy Imaging of Cleared Tissues
- 05:38Results: Neuronal Imaging in Mouse Brain Slice Cleared with Sca/eSF
- 06:42Conclusion
Le protocole fournit une méthode détaillée d’imagerie neuronale dans une tranche de cerveau à l’aide d’une méthode de nettoyage destissus, Sca l eSF. Le protocole comprend la préparation des tissus cérébraux, la clarification des tissus, la manipulation des tranches nettoyées et l’imagerie par microscopie confocale à balayage laser des structures neuronales, des niveaux mésoscopiques aux niveaux microscopiques.
