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1Department of Neuroanatomy,Juntendo University Graduate School of Medicine, 2Department of Cell Biology and Neuroscience,Juntendo University Graduate School of Medicine, 3Advanced Research Institute for Health Sciences,Juntendo University, 4Department of Neuroscience, Graduate School of Medicine,Kyoto University, 5Japan Society for the Promotion of Science, 6Department of Oral Anatomy and Neurobiology, Graduate School of Dentistry,Osaka University, 7Department of Multi-Scale Brain Structure Imaging,Juntendo University Graduate School of Medicine
Chapters
- 00:04Introduction
- 00:57Tissue Clarification
- 02:06Brain Slice Mounting
- 03:52Confocal Laser Scanning Microscopy Imaging of Cleared Tissues
- 05:38Results: Neuronal Imaging in Mouse Brain Slice Cleared with Sca/eSF
- 06:42Conclusion
Protokollet ger en detaljerad metod för neuronal avbildning i hjärnskiva med hjälp av en vävnadsrensningsmetod, ScaleSF. Protokollet inkluderar beredning av hjärnvävnad, vävnadsförtydligande, hantering av rensade skivor och konfokal laserskanningsmikroskopiavbildning av neuronala strukturer från mesoskopiska till mikroskopiska nivåer.
