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Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs
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Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs

Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs

DOI:
10.3791/63943-v

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10:19 min

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August 08, 2022

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, , , ,
1School of Materials Science and Engineering,University of New South Wales, 2School of Chemistry, Australian Centre for NanoMedicine,University of New South Wales, 3School of Biomedical Engineering,University of Sydney

Chapters

  • 00:04Introduction
  • 00:56Bone‐Ink Fabrication
  • 03:12Synthesis of GelMA
  • 04:36Fabrication of Microgel Suspensions for Printing
  • 07:04Printing Bone‐Ink into Cell Suspensions
  • 08:53Results: Printing an Optimized, Nanostructured, Bone‐Mimetic Ceramic Ink Within Cell‐Laden Biomaterials Using COBICS
  • 09:33Conclusion

Summary

자동 번역

자동 번역

This protocol describes a 3D printing technique to fabricate bone-like structures by depositing a calcium phosphate ink in a gelatin-based granular support. Printed bone analogs are deposited in freeform, with flexibility for direct harvesting of the print or crosslinking within a living cell matrix for multiphasic constructs.

Tags

CeramicOmnidirectionalBioprintingCell-ladenSuspensionBone AnalogsAlpha-tricalcium PhosphateGlycerolPolysorbate 80Ammonium Phosphate DibasicBone InkBone RegenerationBone DefectWound Healing3D Printing

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