Journal
/
생화학
/
Self-Assembly of Microtubule Tactoids
JoVE 신문
생화학
This content is Free Access.
JoVE 신문 생화학
Self-Assembly of Microtubule Tactoids

Self-Assembly of Microtubule Tactoids

DOI:
10.3791/63952-v

•

08:49 min

•

June 23, 2022

•

, , , , , , ,
1Physics Department,Syracuse University, 2Department of Molecular Biophysics and Biochemistry,Yale School of Medicine

Chapters

  • 00:04Introduction
  • 00:42Tubulin Preparation
  • 02:10Assembly of Flow Chambers
  • 03:14Tactoid Experiments
  • 04:58Fluorescence Microscopy
  • 06:54Results: Formation of Microtubule Tactoids
  • 08:11Conclusion

Summary

자동 번역

자동 번역

This article presents a protocol for the formation of microtubule assemblies in the shape of tactoids using MAP65, a plant-based microtubule crosslinker, and PEG as a crowding agent.

Tags

Keywords: MicrotubuleSelf-assemblyTactoidsMAP65TubulinRhodamine-labeledFlow ChamberLiquid CrystalSpindle-like Shapes

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/63952?article_type=v

Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
check_url/kr/63952?article_type=v

Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
check_url/kr/63952?article_type=v

Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue
check_url/kr/63952?article_type=v

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella
check_url/kr/63952?article_type=v

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation
check_url/kr/63952?article_type=v

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
check_url/kr/63952?article_type=v

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
check_url/kr/63952?article_type=v

Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria
check_url/kr/63952?article_type=v

Synthesis of Masarimycin, a Small Molecule Inhibitor of Gram-Positive Bacterial Growth
check_url/kr/63952?article_type=v

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

Read Article