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慢病毒介导的shRNA递送到hESC和NPC，使用低成本阳离子聚合物聚乙亚胺（PEI）

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Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI)

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慢病毒介导的shRNA递送到hESC和NPC，使用低成本阳离子聚合物聚乙亚胺（PEI）

DOI:

10.3791/63953-v

•

06:49 min

•

May 24, 2022

•

Muhammad Abid Sheikh, Suraiya Anjum Ansari²

¹Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences,United Arab Emirates University, ²Zayed Center for Health Sciences,United Arab Emirates University

Chapters

00:04Introduction
00:28Transfection of HEK293T Cells Using Either Polymer Polyethylenimine (PEI) or Lipofectamine 3000 Reagent
02:48Lentiviral Vectors (LVS) Collection and Ultracentrifugation
05:07Results: Lentiviral Mediated Delivery of shRNAs in hESCs and NPCs Using Cationic Polymer PEI
06:19Conclusion

Summary

자동 번역

利用低成本的阳离子聚合物聚乙氨基亚胺（PEI），我们生产了慢病毒颗粒，用于H9人胚胎干细胞（hESCs）中shRNA的稳定表达，并高效瞬时转导H9衍生的神经祖细胞（NPC）。

Tags

Keywords: Lentiviral ShRNA HEK293T Transfection Polyethylenimine PEI Cationic Polymer Low Protein Binding Filter Sucrose Cushion Ultracentrifugation DMEM FBS Penicillin Streptomycin Confluency Plasmid DNA Packaging Lipofectamine Virus-containing Media Centrifugation

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