JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Department of Diagnostic Radiology, School of Clinical Medicine, LKS Faculty of Medicine,The University of Hong Kong, 2Department of Oncology, MRC Oxford Institute for Radiation Oncology,University of Oxford, 3Department of Surgery, School of Clinical Medicine, HKU-SZH & LKS Faculty of Medicine,The University of Hong Kong, 4Department of Biomedical Engineering,The Chinese University of Hong Kong, 5Department of Diagnostic and Interventional Radiology,Kwong Wah Hospital
Chapters
- 00:04Introduction
- 01:06Subcutaneous Injection of Human Hepatocellular Carcinoma Cell Lines
- 01:45Orthotopic Liver Implantation and Hepatic Artery Ligation
- 04:00[18F] FMISO and [18F] FDG Injection
- 04:42PET/MR Acquisition
- 05:57Results: Effect of Hepatic Artery Ligation on Orthotopic Hepatocellular Carcinoma in Mice
- 07:02Conclusion
Here, we present a protocol to create orthotopic hepatocellular carcinoma xenografts with and without hepatic artery ligation and perform non-invasive positron emission tomography (PET) imaging of tumor hypoxia using [18F]Fluoromisonidazole ([18F]FMISO) and [18F]Fluorodeoxyglucose ([18F]FDG).
