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Preparation of Human Myocardial Tissue for Long-Term Cultivation
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Preparation of Human Myocardial Tissue for Long-Term Cultivation

Preparation of Human Myocardial Tissue for Long-Term Cultivation

DOI:
10.3791/63964-v

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10:58 min

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June 02, 2022

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, , , , ,
1Walter-Brendel-Centre of Experimental Medicine,University Hospital, LMU Munich, 2German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), 3Division of Experimental Cardiology, Department of Cardiology, Erasmus MC,University Medical Center Rotterdam, 4Institute of Cellular and Molecular Physiology,Friedrich-Alexander-Universität Erlangen-Nürnberg, 5Muscle Research Center Erlangen (MURCE),Friedrich-Alexander-Universität Erlangen-Nürnberg, 6Department of Cardiac Surgery,University Hospital, LMU Munich

Chapters

  • 00:04Introduction
  • 01:01Preparing Agarose and Vibratome
  • 02:01Trimming and Embedding the Samples
  • 03:39Placing the Samples on Cutting Tray and Setting Vibratome
  • 04:17Medium and Incubator Preparation During Slicing Procedure
  • 05:07Preparing and Mounting Slices
  • 07:06Changing Medium
  • 07:51Results: Ex Vivo Cultivation of Human Left Ventricular Myocardial Tissue for Detailed Analysis of Contraction Forces and Kinetics
  • 10:15Conclusion

Summary

자동 번역

자동 번역

We present a protocol for ex vivo cultivation of human ventricular myocardial tissue. It allows for detailed analysis of contraction force and kinetics, as well as the application of pre- and afterload to mimic the in vivo physiological environment more closely.

Tags

Long-term CultivationBiomimetic ChamberStimulation ProtocolsCardiovascular MedicineSlicing BufferAgarose Embedding

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