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1Walter-Brendel-Centre of Experimental Medicine,University Hospital, LMU Munich, 2German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), 3Division of Experimental Cardiology, Department of Cardiology, Erasmus MC,University Medical Center Rotterdam, 4Institute of Cellular and Molecular Physiology,Friedrich-Alexander-Universität Erlangen-Nürnberg, 5Muscle Research Center Erlangen (MURCE),Friedrich-Alexander-Universität Erlangen-Nürnberg, 6Department of Cardiac Surgery,University Hospital, LMU Munich
Chapters
- 00:04Introduction
- 01:01Preparing Agarose and Vibratome
- 02:01Trimming and Embedding the Samples
- 03:39Placing the Samples on Cutting Tray and Setting Vibratome
- 04:17Medium and Incubator Preparation During Slicing Procedure
- 05:07Preparing and Mounting Slices
- 07:06Changing Medium
- 07:51Results: Ex Vivo Cultivation of Human Left Ventricular Myocardial Tissue for Detailed Analysis of Contraction Forces and Kinetics
- 10:15Conclusion
Nous présentons un protocole pour la culture ex vivo de tissu myocardique ventriculaire humain. Il permet une analyse détaillée de la force de contraction et de la cinétique, ainsi que l’application de la pré- et post-charge pour imiter de plus près l’environnement physiologique in vivo .
