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Journal / Biology / 原代培养中分化的小鼠脂肪细胞：胰岛素抵抗模型…
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原代培养中分化的小鼠脂肪细胞：胰岛素抵抗模型
 
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原代培养中分化的小鼠脂肪细胞：胰岛素抵抗模型

Article DOI: 10.3791/63979-v • 09:48 min • February 17th, 2023
February 17th, 2023 •
1, 1, 1, 1, 1
1Instituto de Neurobiología, Universidad Nacional Autónoma de México (UNAM)

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该协议描述了从皮下脂肪中分离小鼠前脂肪细胞，它们分化为成熟脂肪细胞以及诱导胰岛素抵抗。通过蛋白质印迹的胰岛素信号通路成员的磷酸化/激活来评估胰岛素作用。该方法可以直接测定原代脂肪细胞的胰岛素抵抗/敏感性。

Tags

生物学，第192期，前脂肪细胞分离，皮下脂肪，胰岛素作用，TNF-α
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