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1Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases,The Third Affiliated Hospital of Guangzhou Medical University, 2Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, 3School of Biotechnology and Health Sciences,Wuyi University
Chapters
- 00:04Introduction
- 00:51Induced Pluripotent Stem Cells (iPSCs) and EB Preparation (Days 0 1)
- 02:54Soft Light Lamp Preparation (Day 1), EB Transfer, and Medium Replacement (Days 2 5)
- 04:24Neural Induction (Days 5 7)
- 05:25Embedding in the Basement Membrane Matrix (Days 7 10)
- 06:52Organoid Maturation (Days 10 40)
- 07:21Results: Analysis of Cerebral Organoids
- 08:41Conclusion
Zerebrale Organoide bieten beispiellose Möglichkeiten zur Untersuchung der Organentwicklung und der Pathologie menschlicher Krankheiten. Obwohl mit zerebralen Organoid-Kultursystemen große Erfolge erzielt wurden, gibt es immer noch operative Schwierigkeiten bei der Anwendung dieser Technologie. Das vorliegende Protokoll beschreibt ein zerebrales Organoidverfahren, das den Mediumswechsel und den Organoidtransfer erleichtert.
