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1Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases,The Third Affiliated Hospital of Guangzhou Medical University, 2Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, 3School of Biotechnology and Health Sciences,Wuyi University
Chapters
- 00:04Introduction
- 00:51Induced Pluripotent Stem Cells (iPSCs) and EB Preparation (Days 0 1)
- 02:54Soft Light Lamp Preparation (Day 1), EB Transfer, and Medium Replacement (Days 2 5)
- 04:24Neural Induction (Days 5 7)
- 05:25Embedding in the Basement Membrane Matrix (Days 7 10)
- 06:52Organoid Maturation (Days 10 40)
- 07:21Results: Analysis of Cerebral Organoids
- 08:41Conclusion
Los organoides cerebrales proporcionan oportunidades sin precedentes para estudiar el desarrollo de órganos y la patología de enfermedades humanas. Aunque se ha logrado un gran éxito con los sistemas de cultivo de organoides cerebrales, todavía existen dificultades operativas en la aplicación de esta tecnología. El presente protocolo describe un procedimiento organoide cerebral que facilita el cambio medio y la transferencia de organoides.
