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检查骨骼肌中肌内脂肪形成及其细胞起源的指南
JoVE 신문
발생학
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JoVE 신문 발생학
A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle
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检查骨骼肌中肌内脂肪形成及其细胞起源的指南

DOI:
10.3791/63996-v

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09:19 min

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May 26, 2022

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, ,
1Department of Pharmacology and Therapeutics,University of Florida College of Medicine, 2Myology Institute,University of Florida College of Medicine

Chapters

  • 00:05Introduction
  • 00:51Injury of Tibialis Anterior (TA) Muscle
  • 01:28Tissue Harvest
  • 03:43Imaging of Intramuscular Fat
  • 04:26Adipogenic Gene Expression Analysis Using RT-qPCR
  • 07:11Results: Visualization and Quantification of Intramuscular Fat
  • 08:51Conclusion

Summary

자동 번역

자동 번역

用肌内脂肪代替健康的肌肉组织是人类疾病和病症的突出特征。该协议概述了如何可视化，成像和量化肌内脂肪，从而可以严格研究肌内脂肪形成的机制。

Tags

Intramuscular FatSarcopeniaNeuromuscular DiseasesAdipocyte MorphologySkeletal MuscleDuchenne Muscular DystrophyGlycerolTibialis AnteriorHistology

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