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Chapters
- 00:04Preparation of plates
- 01:01Adhesion assay
- 02:40Invasion assay
- 04:04Phenotype observations under microscope
We describe a qualitative assay for yeast adhesion and agar invasion as a measure of invasive and pseudohyphal differentiation. This simple assay can be used to assess the invasive phenotype of various mutants as well as the effects environmental cues and signaling pathways on yeast differentiation.
Tags
AdhesionAgar InvasionS. CerevisiaeBiofilmsIndustrial ProductivityStructuresHuman LifeSustainable EnergyColonizationSignaling PathwaysEnvironmental CuesModel OrganismRas-PKA PathwayKss1 MAPK PathwayHog1 Osmolarity PathwayPseudohyphal FilamentsSignal Transduction ResearchFilamentationPhenotypesMAT Formation AssayColony SpreadSoft AgarCrystal Violet St
