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면역학 및 감염병학

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High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology

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High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology

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High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology

DOI:

10.3791/64012-v

•

03:42 min

•

May 18, 2022

•

Nicholas S. Andresen, Megan K. Wood, Daniela Čiháková, C. Matthew Stewart

¹Department of Otolaryngology – Head & Neck Surgery,Johns Hopkins University School of Medicine, ²W. Harry Feinstone Department of Molecular Microbiology and Immunology,Johns Hopkins University Bloomberg School of Public Health, ³Department of Pathology,Johns Hopkins University School of Medicine

Chapters

00:04Introduction
00:47Temporal Bone Harvest
01:40Tissue Fixation, Decalcification, and Sectioning
02:27Results: Post-Processing Analyses of Middle Ear Tissue
03:02Conclusion

Summary

자동 번역

This article describes a technique for rapid human temporal bone sectioning that utilizes a microsaw with twin diamond blades to generate thin slices for rapid decalcification and analysis of temporal bone immunohistochemistry.

Tags

Temporal Bone Sectioning COVID-19 Middle Ear Pathology Rapid Assessment Otopathology SARS-CoV2 ACE2 Transmembrane Protease Serine Furin Immunohistochemistry

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