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1Daniel Baugh Institute for Functional Genomics and Computational Biology, Department of Pathology, Anatomy, and Cell Biology,Thomas Jefferson University, 2Sidney Kimmel Medical College,Thomas Jefferson University, 3Department of Psychiatry and Behavioral Sciences,Stanford University School of Medicine
Chapters
- 00:04Introduction
- 00:32Running qPCR Chip on a Microfluidic RT‐qPCR Platform
- 02:46Data Analysis
- 06:48Results: Analyzing GLP‐1 Enriched Neuronal Samples
- 08:51Conclusion
The combination of laser capture microdissection and microfluidic RT-qPCR provides anatomic and biotechnical specificity in measuring the transcriptome in single neurons and glia. Applying creative methods with a system's biology approach to psychiatric disease may lead to breakthroughs in understanding and treatment such as the neuroinflammation antireward hypothesis in addiction.
