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一种简单的坑式检测方案,用于可视化和定量体外碎屑病再吸收
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의학
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JoVE 신문 의학
A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
DOI:

07:03 min

June 16, 2022

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Chapters

  • 00:04Introduction
  • 00:51Calcification of 96-Well Cell Culture Plates
  • 01:54Isolation of Peripheral Blood Mononuclear Cells (PBMCs)
  • 02:49Expansion of Osteoclasts Progenitors and Induction of Osteoclastogenesis
  • 04:19Fluorescence Staining of Osteoclasts and Calcium Phosphate Coating
  • 05:17Results: Analysis of Mature Osteoclast Cultured on Calcium Phosphate Coated Wells
  • 06:38Conclusion

Summary

자동 번역

在这里,我们提出了一种简单有效的测定程序,用于使用磷酸钙包被细胞培养板进行再吸收坑测定。

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