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使用单蠕虫数据量化 秀丽隐杆线虫的异质性 - 细菌相互作用

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Using Single-Worm Data to Quantify Heterogeneity in Caenorhabditis elegans-Bacterial Interactions

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使用单蠕虫数据量化秀丽隐杆线虫的异质性 - 细菌相互作用

DOI:

10.3791/64027-v

•

09:54 min

•

July 22, 2022

•

Megan N. Taylor, Satya Spandana Boddu, Nic M. Vega²

¹Department of Biology,Emory University, ²Department of Physics,Emory University

Chapters

00:05Introduction
00:47Preparation and Chemical Permeabilization of Worms
03:41Mechanical Disruption of Individual Worms
07:28Results: Heterogeneous Bacterial Colonization of the C. elegans Intestine
09:30Conclusion

Summary

자동 번역

该协议描述了在冷麻痹和表面漂白以去除外部细菌后，对单个细菌定植 的秀丽隐杆线虫炎的 96孔破坏。将得到的悬浮液接种在琼脂平板上，以便对大量单个蠕虫中的细菌负荷进行准确的中等通量定量。

Tags

Caenorhabditis Elegans Bacterial Interactions Single-worm Data Mechanical Disruption 96-well Format Medium Throughput Quantification Worm Measurements M9TX01 OP50 SDS/DTT Solution Chemical Permeabilization Worm Cuticle

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