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Chapters
- 00:05Introduction
- 00:36Cell Seeding, Maintenance, and Calcification Induction
- 02:13Calcification Detection via Imaging
- 07:51Data Analysis
- 10:13Results: A Semi-Automated Method for In Vitro Calcification Determination
- 11:05Conclusion
Cardiovascular disease is the leading cause of death worldwide. Vascular calcification contributes substantially to the burden of cardiovascular morbidity and mortality. This protocol describes a simple method to quantify vascular smooth muscle cell-mediated calcium precipitation in vitro by fluorescent imaging.
