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厌氧菌小鼠阴道定植模型
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria
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厌氧菌小鼠阴道定植模型

DOI:
10.3791/64032-v

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08:53 min

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May 25, 2022

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, , , , ,
1Division of Biology and Biomedical Sciences,Washington University School of Medicine, 2Department of Obstetrics, Gynecology, and Reproductive Sciences,University of California, 3Glycobiology Research and Training Center,UCSD, 4Department of Pediatrics,Washington University School of Medicine

Chapters

  • 00:05Introduction
  • 00:52Preparation of Inoculum
  • 02:08Vaginal Pre-Lavage and Inoculation with Gardnerella
  • 03:54Dissection and Tissue Homogenization
  • 07:26Results: Enumeration of Bacteria Colonizing Mouse Vagina
  • 08:23Conclusion

Summary

자동 번역

자동 번역

该协议提出了用厌氧培养的人阴道细菌阴道定植的小鼠模型。我们专注于 阴道加德纳菌，同时包括 对双普雷沃氏 菌和核 梭杆菌的建议。该方案也可以用作阴道接种和其他厌氧生长细菌的可行回收的指南。

Tags

Keywords: Anaerobic BacteriaVaginal ColonizationMurine ModelInoculationVaginal LavageColony-forming UnitsObligate AnaerobesFastidious BacteriaSpectrophotometryAnaerobic Conditions

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