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干式大孔海藻酸盐支架的制备和使用用于T细胞的病毒转导
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Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells
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干式大孔海藻酸盐支架的制备和使用用于T细胞的病毒转导

DOI:
10.3791/64036-v

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11:16 min

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September 09, 2022

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, , ,
1Joint Department of Biomedical Engineering,University of North Carolina at Chapel Hill and North Carolina State University, 2Comparative Medicine Institute,North Carolina State University, 3Lineberger Comprehensive Cancer Center,University of North Carolina at Chapel Hill

Chapters

  • 00:10Introduction
  • 00:50Making the Macroporous Alginate Scaffolds
  • 05:47Transduction
  • 10:07Results
  • 10:51Conclusions

Summary

자동 번역

자동 번역

本文是用于创建干燥的大孔藻酸盐支架的方案，该支架介导有效的病毒基因转移，用于T细胞的基因工程，包括用于CAR-T细胞治疗的T细胞。支架被证明以>85%的转导转导活化的原代T细胞。

Tags

Dry Macroporous Alginate ScaffoldsDryduxViral TransductionT CellsSpinoculationRetroNectinCalcium-D-gluconateAlginate SolutionLyophilization48-well Plate24-well Plate

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