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毫秒氢/氘交换质谱法用于生理条件下α-突触核蛋白结构动力学的研究

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Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions

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毫秒氢/氘交换质谱法用于生理条件下α-突触核蛋白结构动力学的研究

DOI:

10.3791/64050-v

•

08:40 min

•

June 23, 2022

•

Neeleema Seetaloo, Jonathan J. Phillips

¹Living Systems Institute, Department of Biosciences,University of Exeter

Chapters

00:04Introduction
00:41Peptide Mapping Procedure
02:38Millisecond Hydrogen/Deuterium Exchange Study
05:34Data Processing and Data Analysis
07:19Results: Measuring the Conformational Dynamics of aSyn
08:14Conclusion

Summary

자동 번역

单体α-突触核蛋白的结构集合影响其生理功能和理化性质。本方案描述了如何进行毫秒氢/氘交换质谱和随后的数据分析，以确定在生理条件下这种内在无序蛋白质的单体的构象信息。

Tags

Alpha-synuclein Hydrogen/deuterium Exchange Mass Spectrometry Intrinsically Disordered Proteins Peptide Mapping Sample Preparation Autosampler HDX Robotics Quench Buffer Peptide Coverage Map Fast HDX Prototype Instrument HDX Data Analysis Software

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