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使用造血细胞因子接种肿瘤的实验性黑色素瘤免疫治疗模型
JoVE 신문
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JoVE 신문 암 연구학
Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine
DOI:

09:15 min

February 24, 2023

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Chapters

  • 00:04Introduction
  • 00:56Preparation of Cultured Tumor Cells for Implantation
  • 02:33Tumor Implantation
  • 03:19Vaccine Preparation and Injection of Flt3L-Expressing B16 F10 (B16-Flt3) Cells
  • 04:20Intratumoral Immune Cell Isolation
  • 06:59Results: Using a Cell-Based Tumor Vaccine Expressing Flt3 Model to Study Intratumoral Immune Cell Infiltrates
  • 08:45Conclusion

Summary

자동 번역

该协议提出了一种癌症免疫治疗模型,使用表达Flt3L的B16-F10黑色素瘤进行基于细胞的肿瘤疫苗接种。该协议演示了程序,包括培养的肿瘤细胞的制备,肿瘤植入,细胞照射,肿瘤生长的测量,肿瘤内免疫细胞的分离和流式细胞术分析。

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