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1Graduate Program in Developmental Biology,Baylor College of Medicine, 2Huffington Center on Aging,Baylor College of Medicine, 3Integrative Program of Molecular and Biochemical Sciences,Baylor College of Medicine, 4Department of Molecular and Human Genetics,Baylor College of Medicine, 5Howard Hughes Medical Institute,Baylor College of Medicine
Chapters
- 00:04Introduction
- 00:45Preparation of Lipid‐Conditioned Bacteria
- 02:00Lipid Supplementation for Caenorhabditis elegans
- 03:37RNA Extraction from a Small Number of Whole Worms
- 05:53Results: Validation of Transcriptional Changes, Transcriptional Evaluations, and Lifespan Assay Upon Lipid Supplementation
- 06:41Conclusion
Le présent protocole décrit des méthodes de supplémentation en lipides dans des cultures liquides et sur plaque pour Caenorhabditis elegans, associées à des études longitudinales et à l’analyse transcriptionnelle des gènes à partir de vers et de tissus de vers en vrac ou de quelques vers.
