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Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy
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JoVE 신문 신경과학
Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy
DOI:

08:49 min

August 01, 2022

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Chapters

  • 00:05Introduction
  • 00:48Light-Sheet Imaging
  • 02:25Image Processing Using NuMorph
  • 04:12Image Analysis
  • 06:26Results: Quantification of Nuclear Markers Within Mouse Brain After Imaging on a Light-Sheet Microscope Using NuMorph
  • 07:57Conclusion

Summary

자동 번역

This protocol describes methods for conducting magnetic resonance imaging, clearing, and immunolabeling of intact mouse brains using iDISCO+, followed by a detailed description of imaging using light-sheet microscopy, and downstream analyses using NuMorph.

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