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用于肿瘤球体半高通量分析的快速光学清除

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Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids

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用于肿瘤球体半高通量分析的快速光学清除

DOI:

10.3791/64103-v

•

08:12 min

•

August 23, 2022

•

Gency Gunasingh, Alexander P. Browning, Nikolas K. Haass

¹The University of Queensland Diamantina Institute,The University of Queensland, ²School of Mathematical Sciences,Queensland University of Technology

Chapters

00:04Introduciton
00:43Preparation of 2% Agarose‐PBS Gel and Clearing Solution
01:39Spheroid Preparation and Staining
04:04Spheroid Mounting and Imaging
05:46Results: Semi-High‐Throughput Imaging of 3D Tumor Spheroids
07:36Conclusion

Summary

자동 번역

肿瘤球体越来越多地用于评估肿瘤细胞 - 微环境相互作用和治疗反应。本方案描述了一种使用快速光学清除对3D肿瘤球体进行半高通量成像的稳健但简单的方法。

Tags

Keywords: Tumor Spheroids 3D Tumor Modeling Optical Clearing Rapid Clearing Semi-high-throughput Analysis Agarose Overlay Method Paraformaldehyde Fixation Antibody Staining Triton X-100 Permeabilization

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