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A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications
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A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

DOI:
10.3791/64123-v

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05:26 min

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September 16, 2022

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, , , , , , , , , , ,
1The Liquid Biopsy and Biomarker Working Module,the Biomedical Research Network in Cancer (CIBERONC)

Chapters

  • 00:05Introduction
  • 01:30Serum Preparation for Liquid Biopsy Studies
  • 02:25Plasma Preparation for Liquid Biopsy Studies
  • 04:08Results: Electrophoresis-Based Analysis of cfDNA Isolated from Plasma
  • 04:28Conclusion

Summary

자동 번역

자동 번역

The liquid biopsy has revolutionized our approach to oncology translational studies, with sample collection, quality, and storage being crucial steps for its successful clinical application. Here we describe a standardized and validated protocol for downstream circulating-free DNA applications that can be applied in most translational research laboratories.

Tags

Liquid BiopsyCirculating Free DNAPreanalytical ProtocolBlood Sample ProcessingPlasma ExtractionSerum ExtractionSample CentrifugationSample StorageHemolysis DetectionStandardized ProcedureTranslational ResearchClinical ApplicationsOncology Research

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