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培养的人间充质干细胞细胞外囊泡的分离、表征和治疗应用
JoVE 신문
생물학
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JoVE 신문 생물학
Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells
DOI:

07:03 min

September 23, 2022

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Chapters

  • 00:05Introduction
  • 01:04Differential Centrifugation for Isolation of Exosomes and Microvesicles
  • 02:25Detection of Particle Numbers and Size Distribution of Extracellular Vesicles
  • 03:29Labeling of Extracellular Vesicles Derived from Mesenchymal Stem Cells
  • 04:50Systemic Injection of Extracellular Vesicles Derived from Mesenchymal Stem Cells
  • 05:24Results: Characterization of Extracellular Vesicles Derived from Mesenchymal Stem Cells
  • 06:19Conclusion

Summary

자동 번역

本协议描述了用于从培养的人MSC中分离和表征代表性EV(外泌体和微囊泡)的差异离心。本文还解释了这些电动汽车的进一步应用。

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