Enkeltpartikel-kryo-EM-dataindsamling med scenehældning ved hjælp af Leginon

Article DOI: 10.3791/64136-v • 04:52 min • July 1st, 2022

July 1st, 2022 •

Sriram Aiyer¹, Timothy S. Strutzenberg¹, Marianne E. Bowman², Joseph P. Noel^2,3, Dmitry Lyumkis^1,4,5

¹Laboratory of Genetics, The Salk Institute for Biological Studies, ²Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, ³Department of Chemistry and Biochemistry, University of California San Diego, ⁴Graduate School of Biological Sciences, Section of Molecular Biology, University of California San Diego, ⁵Department of Integrative Structural and Computational Biology, The Scripps Research Institute

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Denne protokol beskriver et generelt og let at implementere skema til vippet enkeltpartikeldataindsamling i kryo-EM-eksperimenter. En sådan procedure er især nyttig til opnåelse af et EM-kort af høj kvalitet for prøver, der lider af præferenceorienteringsbias på grund af overholdelse af luft-vand-grænsefladen.

Enkeltpartikel-kryo-EM-dataindsamling med scenehældning ved hjælp af Leginon

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