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1Leonard Davis School of Gerontology,University of Southern California, 2Molecular and Computational Biology Department,USC Dornsife College of Letters, Arts and Sciences, 3Biochemistry and Molecular Medicine Department,USC Keck School of Medicine, 4Epigenetics and Gene Regulation,USC Norris Comprehensive Cancer Center, 5USC Stem Cell Initiative
Chapters
- 00:05Introduction
- 01:07Nuclei Isolation
- 04:46Quantify Singlet Nuclei, Debris, and Multiplet Proportion by Flow Cytometry
- 07:35Results: Nuclei Quantification and Purity Assessment by Flow Cytometry
- 08:37Conclusion
Qui presentiamo un protocollo per isolare i nuclei dal cervello del modello di vertebrati di breve durata Nothobranchius furzeri per applicazioni a valle come il sequenziamento dell'RNA a singolo nucleo o il saggio a nucleo singolo per la cromatina accessibile alla trasposasi con sequenziamento (ATAC-seq).
