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工业化、人工智能引导的激光显微切割,用于肿瘤微环境的微尺度蛋白质组学分析
JoVE 신문
암 연구학
This content is Free Access.
JoVE 신문 암 연구학
Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
DOI:

13:01 min

June 03, 2022

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Chapters

  • 00:04Introduction
  • 01:07LMD Slide(s) Preparation
  • 01:58Automated Feature Selection Using Image Analysis Software
  • 06:58Laser Microdissectionvvv
  • 09:41Protein Digestion by Pressure-Cycle Technology (PCT) and Sample Analysis Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
  • 10:55Results: Analysis of the Proteins in LMD-Enriched and Whole Tumor Samples
  • 12:09Conclusion

Summary

자동 번역

该协议描述了一种高通量工作流程,用于人工智能驱动的从染色的薄组织切片图像中分割病理确认感兴趣的区域,以使用激光显微切割富集组织学分辨细胞群。该策略包括一种新颖的算法,能够将表示感兴趣细胞群的分界线直接转移到激光显微镜上。

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