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Chapters
- 00:04Introduction
- 01:05Preparation of Retinal Explants
- 03:08Primate Retinal Explant Culture
- 03:36Fixing and Cryo‐Sectioning
- 05:01Cyclic Guanosine Monophosphate (cGMP) Staining
- 06:13TUNEL Staining
- 07:58cGMP Staining Combined with TUNEL Staining
- 08:40Results: Macaque Monkey Retinal Explant Culture
- 09:20Conclusion
Netvliesexplantaten verkregen uit wilde makaken werden in vitro gekweekt. Retinale degeneratie en de cGMP-PKG signaleringsroute werd geïnduceerd met behulp van de PDE6-remmer zaprinast. cGMP-accumulatie in de explantaten bij verschillende zaprinastconcentraties werd geverifieerd met behulp van immunofluorescentie.
