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Chapters
- 00:05Introduction
- 00:50Cell Culture and Transfection
- 02:28Preparation of Cell Lysates and Loading Samples
- 03:11Preparation of Immunoprecipitants from Cell Lysates
- 04:40Performing the Western Blot
- 06:23Antibody Staining, Image Development, Image Acquisition, and Data Quantification
- 07:16Results: Western Blotting Technique to Study the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2
- 09:25Conclusion
Das vorliegende Protokoll hebt die Anwendung der Western-Blotting-Technik hervor, um die Funktionen und Aktivitäten des neuronalen K-Cl-Co-Transporters KCC2 zu untersuchen. Das Protokoll beschreibt die Untersuchung der KCC2-Phosphorylierung an Kinase-regulatorischen Stellen Thr906/1007 mittels Western Blotting. Außerdem werden zusätzliche Methoden zur Bestätigung der KCC2-Aktivität in diesem Text kurz hervorgehoben.
