Journal
/
신경과학
/
Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation

Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation

DOI:
10.3791/64187-v

•

11:10 min

•

July 06, 2022

•

, , ,
1Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, College of Medicine,Jinan University, 2Department of Clinical Pathology,First Affiliated Hospital of Jinan University, 3Program in Neuroscience, Department of Biomedical Sciences, College of Medicine,Florida State University

Chapters

  • 00:05Introduction
  • 00:50Egg Preparation
  • 02:30Egg Windowing and Embryo Identification
  • 03:39Neural Tube Injection and Electroporation
  • 06:20Otocyst Injection and Electroporation
  • 07:44Results: FMRP Knockdown in the Chicken NM and Auditory Duct and Axonal Tracking of Transfected AG Neurons in the Brainstem
  • 10:18Conclusion

Summary

자동 번역

자동 번역

Using in ovo electroporation, we devised a method to selectively transfect the auditory inner ear and cochlear nucleus in chicken embryos to achieve a cell-group-specific knockdown of fragile X mental retardation protein during discrete periods of circuit assembly.

Tags

Keywords: In Ovo ElectroporationCell-autonomous FunctionFMRPFragile X SyndromeAuditory CircuitStage-specificSite-specificDirection-specificFmr1 KnockdownDrug-inducible VectorEmbryo ManipulationHH StageAlbumen RemovalWindowingInk Injection

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/64187?article_type=v

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
check_url/kr/64187?article_type=v

TMS: Using the Theta-Burst Protocol to Explore Mechanism of Plasticity in Individuals with Fragile X Syndrome and Autism
check_url/kr/64187?article_type=v

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
check_url/kr/64187?article_type=v

In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development
check_url/kr/64187?article_type=v

In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations
check_url/kr/64187?article_type=v

Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury
check_url/kr/64187?article_type=v

A Lightweight, Headphones-based System for Manipulating Auditory Feedback in Songbirds
check_url/kr/64187?article_type=v

Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)
check_url/kr/64187?article_type=v

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents
check_url/kr/64187?article_type=v

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

Read Article