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Identifying Caspases and their Motifs that Cleave Proteins During Influenza A Virus Infection
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Chapters
- 00:04Introduction
- 00:45Assessing the Cleavage of Proteins by Caspases in IAV-Infected Cells
- 02:55Confirmation of Caspase-Mediated Cleavage of Proteins in IAV-Infected Cells by RNA Interference
- 04:13Site-Directed Mutagenesis for Locating the Caspase Cleavage Sites in the Polypeptide
- 05:49Results: Role of Caspases in IAV Infection-Induced Degradation of Cortactin
- 07:32Conclusion
Influenza A virus (IAV) infection activates the caspases that cleave host and viral proteins, which, in turn, have pro- and antiviral functions. By employing inhibitors, RNA interference, site-directed mutagenesis, and western blotting and RT-qPCR techniques, caspases in infected mammalian cells that cleave host cortactin and histone deacetylases were identified.
