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Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis
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Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

DOI:
10.3791/64195-v

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08:19 min

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September 28, 2022

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, , , , , ,
1Key Laboratory of Sustainable Forest Ecosystem Management – Ministry of Education,Northeast Forestry University, 2Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences

Chapters

  • 00:05Introduction
  • 00:33Target Design and In Vitro Synthesis of sgRNA
  • 01:18Embryo Collection and Preparation
  • 02:39Microinjection of Embryos
  • 04:24Post-Injection Insect Rearing
  • 05:07Mutant Screening
  • 06:41Results: Mutant Screening in B. dorsalis
  • 07:39Conclusion

Summary

자동 번역

자동 번역

This paper presents the step-by-step protocols for CRISPR/Cas9 mutagenesis of the Oriental fruit fly Bactrocera dorsalis. Detailed steps provided by this standardized protocol will serve as a useful guide for generating mutant flies for functional gene studies in B. dorsalis.

Tags

Keywords: CRISPR/Cas9MutagenesisOriental Fruit FlyBactrocera DorsalisFunctional Gene ResearchPest ManagementGRNAEmbryo CollectionMicroinjectionCas9 Protein

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