Journal
/
생화학
/
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
JoVE 신문
생화학
Author Produced
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

DOI:
10.3791/64243-v

•

10:43 min

•

July 19, 2022

•

, , , , ,
1Department of Chemical Engineering,Indian Institute of Science, 2Holonyak Micro & Nanotechnology Lab,University of Illinois, Urbana-Champaign, 3Center for BioSystems Science and Engineering,Indian Institute of Science

Chapters

  • 00:04Introduction
  • 00:53Assembly of the Microfluidic Chamber
  • 01:41Making Supported Bilayers on Glass Substrate by Vesicle Fusion
  • 04:22Microscope Setup and Single-Particle Imaging Measurements
  • 07:26Image Acquisition for Counting Subunits in a Protein Assembly
  • 08:40Results: Analyzing the Binding of Cytolysin A (ClyA) Protein and Mobility of DNA Tracer
  • 10:10Conclusion

Summary

자동 번역

자동 번역

Here, a protocol to perform and analyze the binding, mobility, and assembly of single molecules on artificial crowded lipid membranes using single-molecule total internal reflection fluorescence (smTIRF) microscopy is presented.

Tags

Single-molecule ImagingPolymer-crowded Lipid MembranesSupported Lipid BilayersMembrane Biomolecular ReactionsDiffusionAssemblyPOPCDOPE-PEG(2000)Small Unilamellar VesiclesSonicationMicrofluidic Imaging Chambers

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

Read Article