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聚合物拥挤脂质膜上的单分子扩散和组装
JoVE 신문
생화학
Author Produced
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JoVE 신문 생화학
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
DOI:

10:43 min

July 19, 2022

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Chapters

  • 00:04Introduction
  • 00:53Assembly of the Microfluidic Chamber
  • 01:41Making Supported Bilayers on Glass Substrate by Vesicle Fusion
  • 04:22Microscope Setup and Single-Particle Imaging Measurements
  • 07:26Image Acquisition for Counting Subunits in a Protein Assembly
  • 08:40Results: Analyzing the Binding of Cytolysin A (ClyA) Protein and Mobility of DNA Tracer
  • 10:10Conclusion

Summary

자동 번역

在这里,提出了一种使用单分子全内反射荧光(smTIRF)显微镜执行和分析单分子在人工拥挤脂质膜上的结合,迁移率和组装的协议。

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