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Immunology and Infection

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JoVE Journal Immunology and Infection
Use of In Vivo Imaging to Screen for Morphogenesis Phenotypes in Candida albicans Mutant Strains During Active Infection in a Mammalian Host
 

Use of In Vivo Imaging to Screen for Morphogenesis Phenotypes in Candida albicans Mutant Strains During Active Infection in a Mammalian Host

Article DOI: 10.3791/64258-v • 09:24 min • October 12th, 2022
October 12th, 2022 •
1, 1,2, 1
1Department of Pediatrics, Carver College of Medicine, University of Iowa, 2Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa

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This manuscript describes a method for screening moderately sized Candida albicans mutant libraries for morphogenesis phenotypes during active infection in a mammalian host using non-invasive confocal microscopy.

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In Vivo Imaging Morphogenesis Phenotypes Candida Albicans Mutant Strains Active Infection Mammalian Host Environmental Signals In Vitro Model Systems Intradermal Injections Animal Resource Veterinarian Rohan Wakade Post-doctoral Fellow Animal Inoculation Chlorophyll-free Chow YPD Culture Centrifugation DPBS Resuspension Cell Counting
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