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1Department of Microbiology and Immunology,The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, 2Department of Infectious Diseases,The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, 3Victorian Infectious Diseases Service,Royal Melbourne Hospital at the Peter Doherty Institute for Infection and Immunity, 4Department of Infectious Diseases,Alfred Hospital and Monash University, 5Department of Chemical Engineering,The University of Melbourne, 6Department of Biomedical Engineering,The University of Melbourne
Chapters
- 00:05Introduction
- 00:45Preparation
- 01:58Measurement
- 03:53Analysis
- 05:11Determination of the Absolute Fluorescence Intensity per Carrier
- 05:56Cell Experiment
- 07:08Results: A Two-Stream Methodology to Convert Relative, Semiquantitative Flow Cytometry Cell-Carrier Association Data into Absolute Quantification
- 08:17Conclusion
Продемонстрирован рабочий процесс для абсолютной количественной оценки взаимодействий между лекарственными средствами и клетками-носителями с использованием проточной цитометрии, чтобы обеспечить лучшую рациональную оценку новых систем доставки лекарств. Этот рабочий процесс применим к носителям лекарств любого типа.
