Analyzing Starvation-Induced Autophagy in the <em>Drosophila melanogaster</em> Larval Fat Body

00:04Introduction
00:40Drosophila Crossing and Egg Laying
01:54Amino Acid Starvation Induces Autophagy
02:51Third Instar Larvae Dissection and Sample Tissue Processing
04:29Results: The Patterns of GFP‐Atg8a Puncta in Control, Mu1, and Mu2 Clones
05:22Conclusion

Summary

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The present protocol describes the induction of autophagy in the Drosophila melanogaster larval fat body via nutrient depletion and analyzes changes in autophagy using transgenic fly strains.

Analyzing Starvation-Induced Autophagy in the Drosophila melanogaster Larval Fat Body

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