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一种基于流式细胞术的细胞表面蛋白结合测定，用于评估抗癌适配体的选择性和特异性

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A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

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一种基于流式细胞术的细胞表面蛋白结合测定，用于评估抗癌适配体的选择性和特异性

DOI:

10.3791/64304-v

•

10:46 min

•

September 13, 2022

•

Maryam Nakhjavani², Breanna Giles², Mia Strom², Chris Vi², Sahara Attenborough², Sarah Shigdar²

¹School of Medicine,Deakin University, ²Institute for Mental and Physical Health and Clinical Translation, School of Medicine,Deakin University

Chapters

00:05Introduction
01:02Binding Assay
04:10Flow Cytometry and Data Analysis
08:58Results: Flow Cytometric-Based Assay to Study the Binding of an Aptamer and Cancer Cells
09:55Conclusion

Summary

자동 번역

抗癌适配体开发的必要步骤是测试其与靶标的结合。我们展示了一种基于流式细胞术的测定来研究这种结合，强调了包括阴性对照适配体和对该特定蛋白质呈阳性或阴性的癌细胞的重要性。

Tags

Keywords: Flow Cytometry Cell Surface Protein Aptamer Anticancer Selectivity Specificity Immunophenotyping Multi-parametric Cell Culture Trypsinization Cell Counting Cell Stabilization Blocking Buffer Aptamer Folding

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