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Chapters
- 00:04Introduction
- 01:13Preparation of Cell Suspension
- 03:24Live-Dead Analysis of Tumor Spheroids
- 04:05Disintegration and Cell Suspension of Tumor Spheroids
- 05:02Cancer-Associated Fibroblast (CAF) Isolation Through Microbeads
- 06:19Flow Cytometry-Based Analysis of ACTA2 Expression in Isolated CAFs
- 07:28Results: Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts
- 09:30Conclusion
Multicellular 3D tumor spheroids were prepared with lung adenocarcinoma cells, fibroblasts, and monocytes, followed by the isolation of cancer-associated fibroblasts (CAFs) from these spheroids. Isolated CAFs were compared with normal fibroblasts to assess mitochondrial health by studying the mitochondrial transmembrane potential, reactive oxygen species, and enzymatic activities.
