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JoVE Journal
Biochemistry

이 콘텐츠은(는) 무료 액세스입니다.

Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography
 

Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography

Article DOI: 10.3791/64322-v 06:26 min January 6th, 2023
January 6th, 2023

챕터

요약

The present protocol describes a procedure to perform fluorescent size exclusion chromatography (FSEC) on membrane proteins to assess their quality for downstream functional and structural analysis. Representative FSEC results collected for several G-protein coupled receptors (GPCRs) under detergent-solubilized and detergent-free conditions are presented.

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Keywords: Membrane Protein SEC Profile Fluorescence FSEC Purification Solubilization DDM CHS Centrifugation FPLC Size-exclusion Chromatography
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