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人诱导多能干细胞来源的心脏球体的生成、高通量筛选和生物样本库

JoVE 신문
생체공학

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JoVE 신문 생체공학

Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

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人诱导多能干细胞来源的心脏球体的生成、高通量筛选和生物样本库

DOI:

10.3791/64365-v

•

09:23 min

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March 10, 2023

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Renee G. C. Maas, Tess Beekink*¹, Nino Chirico*¹, Christian J. B. Snijders Blok, Inge Dokter, Vasco Sampaio-Pinto, Alain van Mil, Pieter A. Doevendans, Jan W. Buikema, Joost P. G. Sluijter*¹, Francesca Stillitano*¹

¹Utrecht Regenerative Medicine Center, Circulatory Health Laboratory, University Utrecht, Department of Cardiology,University Medical Center Utrecht, ²Amsterdam Cardiovascular Sciences, Department of Physiology,Amsterdam University Medical Center

Chapters

00:04Introduction
00:28Cardiac Spheroid Generation
02:40Cryopreservation of Cardiac Spheroids (CSs)
03:58Thawing of Cardiac Spheroids
04:58Assessment of Intracellular Ca²⁺ Transients
06:18Results: Generation and Cryopreservation of Cardiac Spheroids from hiPSC‐CMs
08:34Conclusion

Summary

자동 번역

这里介绍的是一组方案，用于从以高通量、多维形式培养的人诱导多能干细胞衍生心肌细胞中生成和冷冻保存心脏球体（CS）。该三维模型可作为疾病建模、高通量筛选的强大平台，并在冷冻保存后保持其功能。

Tags

Keywords: Cardiac Spheroids Human-induced Pluripotent Stem Cells Cardiomyocytes High-throughput Screening Biobanking Cell Culture Cell Dissociation Cell Counting Spheroid Formation Spheroid Maintenance Cryopreservation

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